Application of a Peptide-Mediated Magnetic Separation-Phage Assay for Detection of Viable Mycobacterium avium subsp paratuberculosis to Bovine Bulk Tank Milk and Feces Samples

作者:Foddai Antonio; Strain Samuel; Whitlock Robert H; Elliott Christopher T; Grant Irene R*
来源:Journal of Clinical Microbiology, 2011, 49(5): 2017-2019.
DOI:10.1128/JCM.00429-11

摘要

Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.

  • 出版日期2011-5