Diallyl trisulfide suppresses dextran sodium sulfate-induced mouse colitis: NF-kappa B and STAT3 as potential targets

作者：Lee Hyung Jun; Lee Hee Geum; Choi Ki Seok; Surh Young Joon; Na Hye Kyung^*

来源：Biochemical and Biophysical Research Communications, 2013, 437(2): 267-273.

DOI：10.1016/j.bbrc.2013.06.064

• Diallyl trisulfide
• NE-kappa B
• STAT3
• Mouse colitis

Diallyl trisulfide (DATS), one of the volatile constituents of garlic oil, has been reported to possess antioxidant, anti-inflammatory, and anti-carcinogenic properties. In this study, DATS (10 mu mop given orally for 7 days before and for another 7 days after starting administration of 2.5% dextran sulfate sodium (DSS) in drinking water protected against colitis induced by DSS in male ICR mice. DATS significantly inhibited the DSS-induced DNA binding of NF-kappa B, phosphorylation of I kappa B alpha and the expression of pro-inflammatory proteins, such as cyclooxygenase-2 and inducible nitric oxide synthase, which are major target proteins of NF-kappa B. The DSS-induced DNA binding and phosphorylation at the Tyr 705 residue of signal transducer and activator of transcription 3 (STAT3), and expression of its major target protein cyclin D1 in mouse colonic mucosa were also attenuated by DATS administration. Likewise, DSS-induced phosphorylation of extracellular signal-regulated kinase 1/2 was suppressed by DATS treatment. In conclusion, DATS ameliorates the DSS-induced mouse colitis presumably by blocking inflammatory signaling mediated by NF-kappa B and STAT3.

• 出版日期2013-7-26