Molecular characterization, tissue distribution and kinetic analysis of carnitine,palmitoyltransferase I in juvenile yellow catfish Pelteobagrus fulvidraco

作者：Zheng, Jia-Lang; Luo, Zhi^*; Zhu, Qing-Ling; Chen, Qi-Liang; Gong, Yuan

来源：Genomics, 2013, 101(3): 195-203.

DOI：10.1016/j.ygeno.2012.12.002

• Pelteobagrus fulvidraco
• Carnitine palmitoyltransferase I
• Isoforms
• mRNA expression
• Kinetic characteristics

Up to to date, only limited information is available on genetically and functionally different isoforms of CPT I enzyme in fish. In the study, molecular characterization and their tissue expression profile of three CPT let isoforms (CPT I alpha 1 alpha, CPT I alpha 1b and CPT I alpha 2a) and a CPT I beta isoform from yellow catfish Pelteobagrus fulvidraco is determined. The activities and kinetic features of CPT I from several tissues have also been analyzed. The four CPT I isoforms in yellow catfish present distinct differences in amino acid sequences and structure. They are widely expressed in liver, heart, white muscle, spleen, intestine and mesenteric adipose tissue of yellow catfish at the mRNA level, but with the varying levels. CPT I activity and kinetics show tissue-specific differences stemming from co-expression of different isoforms, indicating more complex pathways of lipid utilization in fish than in mammals, allowing for precise control of lipid oxidation in individual tissue.

• 出版日期2013-3
• 单位华中农业大学