GRP78 positively regulates estrogen-stimulated cell growth mediated by ER-α36 in gastric cancer cells

作者：Fu, Zhengqi; Wang, Xuming; Zhou, Hongyan; Li, Yan; Chen, Ying; Wang, Zhaoyi; Liu, Lijiang^*

来源：Molecular Medicine Reports, 2017, 16(6): 8329-8334.

DOI：10.3892/mmr.2017.7615

• 78 kDa glucose-regulated protein
• estrogen signaling
• estrogen receptor-alpha 36
• cyclin D1
• gastric cancer

Estrogen receptor (ER)-alpha 36, a novel isoform of ER, primarily mediates non-classical estrogen signaling. It has been reported that ER-alpha 36-mediated growth stimulating signals are involved in the malignancy of gastric tumor cells. However, the mechanism underlying the regulation of ER-alpha 36 function in development of gastric cancer remains to be elucidated. The present study investigated the role of 78 kDa glucose-regulated protein (GRP78) in the regulation of ER-alpha 36 expression and signaling during the growth of gastric tumor cells. It was demonstrated that GRP78 expression was detectable in gastric cancer tumor tissues, and was positively-correlated with tumor stage, lymphatic metastasis and ER-alpha 36 expression (P<0.05). An increased growth rate, and increased expression of ER-alpha 36 and the cell cycle regulator cyclin D1 was detected in cells with GRP78 overexpression (SGC-High78 cells). SGC-High78 cells are more sensitive to estrogen compared with SGC-Control cells. Therefore, the results of the present study demonstrated that GRP78 positively regulated ER-alpha 36 expression and signaling with cell growth in gastric cancer, which is involved in gastric carcinogenesis.

• 出版日期2017-12
• 单位江汉大学