Analysis of Four Cephalosporins in Milk by Capillary Electrochromatography with O-Cynaoethyl Chitosan-Coated Open Tubular Column

作者：Lu, Aixing; Li, Qingyin; Lu, Haixia^*; Yu, Jianchang

来源：Separation Science and Technology (Philadelphia), 2015, 50(6): 859-864.

DOI：10.1080/01496395.2014.976874

• O-cynaoethyl chitosan
• cephalosporins
• milk
• open-tubular capillary electrochromatography

Open-tubular capillary electrochromatography (OT-CEC) with a new stationary phase, O-cynaoethyl chitosan, has been applied to the separation of four cephalosporins. Several experimental parameters such as the buffer type, pH and buffer concentration, mobile phase composition, and applied voltage were evaluated. Baseline resolution of the studied cephalosporins could be achieved within 14 min. The calibration curves of cefixime, cephalothin, cefuroxime, and cefoperazone were linear over the concentration range of 0.6-50, 0.8-50, 0.8-50, and 1.0-50 mu g/mL, respectively. The detection limits (S/N = 3) of four cephalosporins ranged from 0.2 to 0.4 mu g/mL and the recoveries of the milk samples were about 82.0-96.7% (RSD <= 3.8%).

• 出版日期2015-4-13
• 单位福州大学