Development of quantitative HPTLC-densitometry methods following a model approach for transfer of TLC screening methods for pharmaceutical products of atenolol, chloramphenicol, furosemide, glibenclamide, penicillin V potassium, and praziquantel

作者：Zeng Bingsong; Nguyen Kaitlin; Sherma Joseph^*

来源：Journal of Liquid Chromatography & Related Technologies, 2018, 41(6): 324-328.

DOI：10.1080/10826076.2018.1448689

• Amitriptyline HCL
• amodiaquine
• atenolol
• chloramphenicol
• diphenhydramine HCL
• furosemide
• glibenclamide
• mebendazole
• model process for tlc to quantitative hptlc transfer
• penicillin V potassium
• praziquantel
• thin-layer chromatography

Transfer of six thin-layer chromatography (TLC) Global Pharma Health Fund E.V. Minilab manual protocols for detecting fake drugs in pharmaceutical products to quantitative high-performance TLC (HPTLC)-densitometry methods was performed following a previously published model process. The developed and validated methods for tablets or capsules containing atenolol, chloramphenicol, furosemide, glibenclamide, penicillin V potassium, and praziquantel involved use of a limited list of inexpensive, relatively nontoxic, readily available solvents and other reagents; silica gel 60F(254) plates; automated bandwise sample and standard solution application; ascending mobile phase development of plates in a chamber; and automated slit scanning densitometry for detection, identification, and quantification. Validation data for methods developed in an early version of the transfer model process that did not include standard addition validation are reported for pharmaceutical products containing amitriptyline HCl, amodiaquine, diphenhydramine HCl, and mebendazole.
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• 出版日期2018