A Second-Generation Phosphohistidine Analog for Production of Phosphohistidine Antibodies

作者：Kee Jung Min; Oslund Rob C; Couvillon Anthony D; Muir Tom W^*

来源：Organic Letters, 2015, 17(2): 187-189.

DOI：10.1021/ol503320p

Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.

• 出版日期2015-1-16