Micropatterned co-cultures of T-lymphocytes and epithelial cells as a model of mucosal immune system

作者：Stybayeva Gulnaz; Zhu He; Ramanculov Erlan; Dandekar Satya; George Michael^*; Revzin Alexander

来源：Biochemical and Biophysical Research Communications, 2009, 380(3): 575-580.

DOI：10.1016/j.bbrc.2009.01.164

• Antibody microarrays
• Micropatterned co-cultures
• Mucosal immune system
• T-lymphocytes
• Epithelial cells
• HIV enteropathy

Gut-associated lymphoid tissue is a major target and reservoir of human immunodeficiency virus (HIV)infected T-cells. Our studies seek to recapitulate, in vitro, interactions between HIV-infected T-lymphocytes and intestinal epithelial cells in order to investigate the mechanisms underlying the disruption of normal epithelial cell and barrier function. Here, we describe a novel approach for creating co-cultures of healthy or HIV-infected T-lymphocytes (Jurkat) and human intestinal epithelial (HT-29) cells where both cell types are positioned on the same surface in a price spatial configuration (micropattern). This co-culture method simplified observation/monitoring of the two cell types and was particularly suited for laser microdissection-based retrieval of the desired cells for downstream gene expressions Studies. DNA microarray analysis of epithelial cells retrieved from co-cultures with HIV-1-infected vs. uninfected Jurkat cells revealed that epithelial cells from HIV-infected co-cultures exhibited gene expression patterns consistent with disruption of epithelial barrier formation. Overall, the micropatterned co-culture system described here is envisioned as a valuable new tool for delineating how HIV and other infections contribute to dysfunction of mucosal epithelium.

• 出版日期2009-3-13