An HPLC-DPPH method for antioxidant activity from sugarcane molasses

作者：Chen Mingshun; Zhao Yi; Chen Fuquan; Zhao Zhengang^*; Yu Shujuan; Abbasi Arshad Mehmood

来源：Zuckerindustrie, 2015, 140(10): 632-639.

• sugarcane molasses
• ultrasonic-assisted extraction
• phenolic compounds
• response surface methodology
• HPLC-DPPH

Sugarcane molasses is potentially rich in health-promoting phenolic compounds. Present study was designed to optimize experimental conditions for ultrasonic-assisted extraction of antioxidant compounds from sugarcane molasses using response surface methodology. An HPLCDPPH method for simultaneous determination of antioxidant activity was also developed. Ethanol concentration 80-84% (v/v), temperature 58-59 degrees C, and 47-50 min time duration were the most favorable conditions. In these conditions, the optimal experimental results were total phenolic content 18 mg gallic acid equivalents/g, with DPPH free radical scavenging ability of 92%, which was same as the predicted values by RSM model. Catechin, vanillic acid, isorhamnetin-3-O-glucoside, eugenol, schaftoside, diosmetin-7-O-beta-D-glucopyranoside, ferulic acid, and caffeoylquinic acid were identified using HPLC-MS/MS. Among the antioxidant compounds, schaftoside was the most abundant antioxidant (92.081 mu g/g dry substance), while ferulic acid exhibited the highest antioxidant activity. These results suggest that HPLC-DPPH method is more specific, accurate and less time consuming and can sever as a quality control tool.

• 出版日期2015-10
• 单位华南理工大学