Deficient Mechanical Activation of Anabolic Transcripts and Post-Traumatic Cartilage Degeneration in Matrilin-1 Knockout Mice

作者：Chen, Yupeng^*; Cossman, Jack; Jayasuriya, Chathuraka T.; Li, Xin; Guan, Yingjie; Fonseca, Vera; Yang, Kun; Charbonneau, Cherie; Yu, Hongchuan; Kanbe, Katsuaki; Ma, Peter; Darling, Eric; Chen, Qian^*

来源：PLos One, 2016, 11(6): e0156676.

DOI：10.1371/journal.pone.0156676

• ATOMIC-FORCE MICROSCOPY
• MATRIX PROTEIN
• CHONDROCYTE DIFFERENTIATION
• COLLAGEN-II
• IN-SITU
• OSTEOARTHRITIS
• ORGANIZATION
• PROGRESSION
• MATURATION
• AGGRECAN

Matrilin-1 (Matn1), a cartilage-specific peri-cellular and extracellular matrix (ECM) protein, has been hypothesized to regulate ECM interactions and transmit mechanical signals in cartilage. Since Matn1 knock-out (Matn1(-/-)) mice exhibit a normal skeleton, its function in vivo is unclear. In this study, we found that the anabolic Acan and Col2a transcript levels were significantly higher in wildtype (Matn1(+/+)) mouse cartilage than that of MATN1(-/-) mice in vivo. However, such difference was not observed between Matn1(+/+) and MATN1(-/-) chondrocytes cultured under stationary conditions in vitro. Cyclic loading significantly stimulated Acan and Col2a transcript levels in Matn1(+/+) but not in MATN1(-/-) chondrocytes. This suggests that, while Matn1(+/+) chondrocytes increase their anabolic gene expression in response to mechanical loading, the MATN1(-/-) chondrocytes fail to do so because of the deficiency in mechanotransduction. We also found that altered elastic modulus of cartilage matrix in Matn1(-/-) mice, suggesting the mechanotransduction has changed due to the deficiency of Matn1. To understand the impact of such deficiency on joint disease, mechanical loading was altered in vivo by destabilization of medial meniscus. While Matn1(+/+) mice exhibited superficial fissures and clefts consistent with mechanical damage to the articular joint, Matn1(-/-) mice presented more severe cartilage lesions characterized by proteoglycan loss and disorganization of cells and ECM. This suggests that Matn1 deficiency affects pathogenesis of post-traumatic osteoarthritis by failing to up-regulate anabolic gene expression. This is the first demonstration of Matn1 function in vivo, which suggests its protective role in cartilage degeneration under altered mechanical environment.

• 出版日期2016-6-7
• 单位西安交通大学