A single quantum dot-based nanosensor for the signal-on detection of DNA methyltransferase

作者:Ma, Fei; Liu, Wen-jing; Tang, Bo*; Zhang, Chun-yang*
来源:Chemical communications, 2017, 53(51): 6868-6871.
DOI:10.1039/c7cc03736h

摘要

We develop a single quantum dot ( QD)-based nanosensor for the signal-on detection of DNA methyltransferase ( MTase). By integration of single-molecule counting with the QD-based fluorescence resonance energy transfer ( FRET), the proposed nanosensor can sensitively detect DNA MTase with a detection limit of as low as 0.002 U mL(-1), and it can be further applied for inhibitor screening and accurate detection of DNA MTase in complex biological samples.