Associations of vitamin D status and vitamin D-related polymorphisms with sex hormones in older men

作者：Rafiq R^*; van Schoor N M; Sohl E; Zillikens M C; Oosterwerff M M; Schaap L; Lips P; de Jongh R T

来源：Journal of Steroid Biochemistry and Molecular Biology, 2016, 164: 11-17.

DOI：10.1016/j.jsbmb.2015.11.013

• Vitamin D
• Vitamin D receptor
• Polymorphisms
• Sex hormones
• Gonadotropins

Objective: Evidence regarding relationships of serum 25-hydroxyvitamin D (25(OH)D) with sex hormones and gonadotropin concentrations remains inconsistent. Polymorphisms in vitamin D-related genes may underly these relationships. Our aim was to examine the relationship of vitamin D status and polymorphisms in vitamin D-related genes with sex hormone and gonadotropin levels.
Design and measurements: We analysed data from the Longitudinal Aging Study Amsterdam, an ongoing population-based cohort study of older Dutch individuals (65-89 years). We included data of men with measurements of serum 25-hydroxyvitamin D (25(OH)D) (n = 643) and determination of vitamin D related gene polymorphisms (n=459). 25(OH)D concentrations were classified into four categories: <25, 25-50, 50-75 and >75 nmol/L. Outcome measures were total testosterone, calculated bioavailable and free fraction testosterone, SHBG, estradiol, LH and FSH concentrations. Hypogonadism was defined as a total testosterone level <8.0 nmol/L.
Results: Serum 25(OH)D was positively associated with total and bioavailable testosterone levels. After adjustments for confounders, men with serum 25(OH)D less than 25 (n = 56), 25-50 (n = 199) and 5075 nmol/L (n=240) had lower total testosterone levels compared to men with serum 25(OH)D higher than 75 nmol/L (n = 148) (beta (95% confidence interval): -2.1 (-3.7 to -0.4 nmol/L), -0.8 (-1.9 to 0.4 nmol/L) and -1.4 (-2.4 to -0.3 nmol/L), respectively). For bioavailable testosterone the association was significant only for men with serum 25(OH)D less than 25 nmol/L (-0.8 (-1.4 to -0.1 nmol/L)) compared to men with serum 25(OH)D >75 nmol/L. Serum 25(OH)D was not related to SHBG, estradiol or gonadotropin levels. Hypogonadism (n = 29) was not associated with lower serum 25(OH)D. No significant differences were found in hormone levels between the different genotypes of the vitamin D related gene polymorphisms. Also, the polymorphisms did not modify the relationships of serum 25(OH) D with sex hormones or gonadotropins.
Conclusion: Vitamin D status is positively associated with testosterone levels. No association was found between vitamin D-related gene polymorphisms and hormone levels.

• 出版日期2016-11