Determination of thermodynamic pK(a) values of pharmaceuticals from five different groups using capillary electrophoresis

作者：Zrncic Mirta; Babic Sandra^*; Pavlovic Dragana Mutavdzic

来源：Journal of Separation Science, 2015, 38(7): 1232-1239.

DOI：10.1002/jssc.201401057

• Acid dissociation constants
• Capillary electrophoresis
• Pharmaceuticals

A determination of the thermodynamic acid dissociation constants (pK(a)) of 22 frequently used pharmaceuticals using capillary electrophoresis in aqueous media is presented in this work. The investigated pharmaceuticals belong to different pharmacological groups: macrolides, fluoroquinolones, sulfonamides, -lactams, tetracyclines, and other miscellaneous pharmaceuticals. The electrophoretic mobilities of the investigated analytes were monitored in a pH range from 2.00 to 10.82. The data were fitted with an appropriate mathematical model using a nonlinear regression analysis to obtain pK(a) values. Experimentally obtained data were well described by the mathematical model chosen for each analyte that was confirmed by r(2) values higher than 0.99 for most of the investigated analytes. Extrapolations to zero ionic strength were used to determine the thermodynamic pK(a) values. Experimentally obtained acid dissociation constants were interpreted using structural formulae of investigated analytes and the moieties corresponding to specific pK(a) were identified.

• 出版日期2015-4