Altered Expression of Pro-inflammatory Cytokines in Ovarian Follicles of Cows with Cystic Ovarian Disease

作者：Baravalle M E; Stasse A E; Velazquez M M L; Belotti E M; Rodriguez F M; Ortega H H; Salvetti N R^*

来源：Journal of Comparative Pathology, 2015, 153(2-3): 116-130.

DOI：10.1016/j.jcpa.2015.04.007

• cow
• cytokine
• immunohistochemistry
• ovarian cyst

A growing body of evidence suggests that ovulation shares many of the features of an inflammatory reaction and that cytokines play many diverse and important roles in reproductive biology. The aim of this study was to examine the expression of the pro-inflammatory cytokines interleukin (IL)-1 alpha, IL-6 and tumour necrosis factor (TNF)-alpha in ovarian cells from cows with cystic ovarian disease (COD) as compared with that in ovarian structures from regularly cycling cows. Expression of genes encoding IL-1 alpha, IL-6 and TNF-alpha. was detected by real-time polymerase chain reaction in follicular cells from ovaries from healthy cows and cows with COD with no significant differences. However, immunohistochemistry showed increased expression of IL-1 alpha, IL-6 and TNF-alpha in cystic follicles, suggesting that this expression may be related to the persistence of follicular cysts. The effect of COD was evident for IL-1 alpha and TNF-alpha, and a follicular structure-disease interaction was observed in the expression of all the cytokines evaluated. Thus, altered expression of these proinflammatory cytokines may be related to ovulation failure and development of follicular cysts.

• 出版日期2015-10