Antibody-independent, CD4(+) T-Cell-Dependent protection against pneumococcal colonization elicited by intranasal immunization with purified pneumococcal proteins

作者：Basset Alan; Thompson Claudette M; Hollingshead Susan K; Briles David E; Ades Edwin W; Lipsitch Marc; Malley Richard^*

来源：Infection and Immunity, 2007, 75(11): 5460-5464.

DOI：10.1128/IAI.00773-07

Immunity to pneumococcal colonization in mice by exposure to live or killed pneumococci has been shown to be antibody independent but dependent on CD4(+) T cells. Here we show that intranasal immunization with pneumococcal proteins (pneumococcal surface protein C, adhesin A, and a pneumolysoid) can elicit a similar mechanism of protection. Colonization could be significantly reduced in mice congenitally deficient in immunoglobulins after intranasal immunization with this mixture of proteins; conversely, the depletion of CD4(+) T cells in immunized wild-type mice at the time of challenge eliminated the protection afforded by immunization. Overall, our results show that intranasal immunization with a mixture of pneumococcal proteins protects against colonization in an antibody-independent, CD4(+) T-cell-dependent manner.

• 出版日期2007-11