Use of Gifu Anaerobic Medium for culturing 32 dominant species of human gut microbes and its evaluation based on short-chain fatty acids fermentation profiles

作者：Gotoh Aina; Nara Misaki; Sugiyama Yuta; Sakanaka Mikiyasu; Yachi Hiroyuki; Kitakata Aya; Nakagawa Akira; Minami Hiromichi; Okuda Shujiro; Katoh Toshihiko; Katayama Takane; Kurihara Shin^*

来源：Bioscience Biotechnology and Biochemistry, 2017, 81(10): 2009-2017.

DOI：10.1080/09168451.2017.1359486

• gut microbes
• standard medium
• short-chain fatty acids
• dominant human gut bacteria
• Gifu anaerobic medium

Recently, a "human gut microbial gene catalogue," which ranks the dominance of microbe genus/species in human fecal samples, was published. Most of the bacteria ranked in the catalog are currently publicly available; however, the growth media recommended by the distributors vary among species, hampering physiological comparisons among the bacteria. To address this problem, we evaluated Gifu anaerobic medium (GAM) as a standard medium. Forty-four publicly available species of the top 56 species listed in the "human gut microbial gene catalogue" were cultured in GAM, and out of these, 32 (72%) were successfully cultured. Short-chain fatty acids from the bacterial culture supernatants were then quantified, and bacterial metabolic pathways were predicted based on in silico genomic sequence analysis. Our system provides a useful platform for assessing growth properties and analyzing metabolites of dominant human gut bacteria grown in GAM and supplemented with compounds of interest.

• 出版日期2017