Mass spectrometric determination of the quantity and enzyme activity of cytochromes P450

作者：Moskaleva N E^*; Zgoda V G; Archakov A I

来源：Russian Journal of Bioorganic Chemistry, 2011, 37(2): 131-145.

DOI：10.1134/S1068162011010122

• quantitative proteomics
• cytochromes P450
• measurement of isoform content
• label-free method
• mass spectrometry

Concentrations of selected members of cytochrome P450 1A, 3A, 1E, 2C, and 2D subfamilies were measured on a triple quadrupole mass spectrometer using the method of multiple reaction monitoring (MRM). The procedure was developed and tested on samples of mouse liver microsomes from control and phenobarbital or methylcholanthrene-induced groups. The procedure enabled the mass spectrometric quantitation of individual P450 isoforms without using isotopic labels or chemical derivatization. Quantitation results for certain P450 isoforms correlated with the changes in enzyme activity measured using isoform-specific marker substrates.

• 出版日期2011-3