An interaction between alpha 7 nicotinic receptors and a G-protein pathway complex regulates neurite growth in neural cells

作者：Nordman Jacob C; Kabbani Nadine^*

来源：Journal of Cell Science, 2012, 125(22): 5502-5513.

DOI：10.1242/jcs.110379

• Gprin
• Axon growth
• Proteomics
• GAP-43
• Cytoskeleton
• Nicotine
• Neurite
• Bungarotoxin
• Growth cone

The alpha 7 acetylcholine nicotinic receptor (alpha 7) is an important mediator of cholinergic transmission during brain development. Here we present an intracellular signaling mechanism for the alpha 7 receptor. Proteomic analysis of immunoprecipitated alpha 7 subunits reveals an interaction with a G protein pathway complex (GPC) comprising G alpha(i/o), GAP-43 and G protein regulated inducer of neurite outgrowth 1 (Gprin1) in differentiating cells. Morphological studies indicate that alpha 7 receptors regulate neurite length and complexity via a Gprin1-dependent mechanism that directs the expression of alpha 7 to the cell surface. alpha 7-GPC interactions were confirmed in embryonic cortical neurons and were found to modulate the growth of axons. Taken together, these findings reveal a novel intracellular pathway of signaling for alpha 7 within neurons, and suggest a role for its interactions with the GPC in brain development.

• 出版日期2012-11-15