Differential expression of steroidogenic factors 1 and 2, cytochrome P450scc, and steroidogenic acute regulatory protein in human pancreas

作者：Morales Angelica^*; Vilchis Felipe; Chavez Bertha; Morimoto Sumiko; Chan Carlos; Robles Diaz Guillermo; Diaz Sanchez Vicente

来源：Pancreas, 2008, 37(2): 165-169.

• normal human pancreas
• steroidogenic factor 1 (NR5A1)
• steroidogenic factor 2 (NR5A2)
• cytochrome P450scc (CYP11A1)
• steroidogenic acute regulatory (StAR) protein
• steroidogenesis

Objectives: The aim of this study was to investigate the expression of the 4 gene transcripts, steroidogenic factors 1 (SF-1) and 2 (SF-2), steroidogenic acute regulatory (StAR), and cytochrome P450 11A1, involved in the synthesis of steroid hormones in normal human pancreas.
Methods: Total RNA was extracted from normal male (n = 5) and female (n = 5) samples, obtained from the organ donor program. The expression levels of SF-1, SF-2, StAR protein, and P450scc were assessed by reverse transcription-polymerase chain reaction and complemented with immunohistochemistry analysis.
Results: Polymerase chain reaction products amplification for all genes was present in both male and female samples, although differential expression was observed. The signals detected were much more evident in male than in female messenger RNA isolates for SF-1, SF-2, and StAR protein. The expression for P450scc was more intense in female samples. A similar pattern was observed in the immunohistochemical studies.
Conclusions: Normal human pancreas expresses 4 gene transcripts involved in steroid synthesis similarly to steroidogenic organs. A distinctive characteristic is the sexually dimorphic expression of these factors. These data provide further evidence to support that the pancreas is a truly steroidogenic tissue, highlighting the presence of sex- and location-related differences in the expression of steroidogenic factors.

• 出版日期2008-8