Monitoring bisphenol A and estrogenic chemicals in thermal paper with yeast-based bioreporter assay

作者：Rajasarkka Johanna^*; Koponen Jani; Airaksinen Riikka; Kiviranta Hannu; Virta Marko

来源：Analytical and Bioanalytical Chemistry, 2014, 406(23): 5695-5702.

DOI：10.1007/s00216-014-7812-x

• Bioavailability
• Bioreporter
• Bisphenol A
• Endocrine-disrupting compounds
• Saccharomyces cerevisiae
• Thermal paper

Bioluminescent Saccharomyces cerevisiae yeast-based bioreporters were used to monitor bisphenol A and other estrogenic chemicals in thermal paper samples collected mainly from Finland on two occasions in 2010/2011, and 2013. The bisphenol A-targeted (BPA-R) and the human oestrogen receptor (hER alpha) bioreporters were applied to analyse both non-treated and extracted paper samples. Bisphenol A was readily bioavailable to the yeast bioreporters on the non-treated paper samples without any pre-treatment. Detected concentrations ranged from a detection limit of 9-142 mu g/g to over 20 mg/g of bisphenol A equivalents in the thermal papers. Low bisphenol A like activities were detected in many samples, and were considered to be caused by residual bisphenol A or other types of bisphenols, such as bisphenol S. Most of the thermal paper samples were toxic to the yeast bioreporters. The toxicity did not, however, depend on the bisphenol A concentration of the samples. The yeast bioreporters were demonstrated to be a robust and cost-efficient method to monitor thermal paper samples for their bisphenol A content and estrogenicity. Thermal paper was considered as a potential BPA source for both human exposure and environmental emission.

• 出版日期2014-9