Activation of maxi-anion channel by protein tyrosine dephosphorylation

作者：Toychiev Abduqodir H; Sabirov Ravshan Z; Takahashi Nobuyaki; Ando Akatsuka Yuhko; Liu Hongtao; Shintani Takafumi; Noda Masaharu; Okada Yasunobu^*

来源：American Journal of Physiology - Cell Physiology, 2009, 297(4): C990-C1000.

DOI：10.1152/ajpcell.00131.2009

• phosphorylation/dephosphorylation
• protein tyrosine phosphatase
• fibroblast

Toychiev AH, Sabirov RZ, Takahashi N, Ando-Akatsuka Y, Liu H, Shintani T, Noda M, Okada Y. Activation of maxi-anion channel by protein tyrosine dephosphorylation. Am J Physiol Cell Physiol 297: C990-C1000, 2009. First published August 5, 2009; doi:10.1152/ajpcell.00131.2009.-The maxi-anion channel with a large single-channel conductance of >300 pS, and unknown molecular identity, is functionally expressed in a large variety of cell types. The channel is activated by a number of experimental maneuvers such as exposing cells to hypotonic or ischemic stress. The most effective and consistent method of activating it is patch membrane excision. However, the activation mechanism of the maxi-anion channel remains poorly understood at present. In the present study, involvement of phosphorylation/dephosphorylation in excision-induced activation was examined. In mouse mammary fibroblastic C127 cells, activity of the channel was suppressed by intracellular application of Mg-ATP, but not Mg-5'-adenylylimidodiphosphate (AMP-PNP), in a concentration-dependent manner. When a cocktail of broad-spectrum tyrosine phosphatase inhibitors was applied, channel activation was completely abolished, whereas inhibitors of serine/threonine protein phosphatases had no effect. On the other hand, protein tyrosine kinase inhibitors brought the channel out of an inactivated state. In mouse adult skin fibroblasts (MAFs) in primary culture, similar maxi-anion channels were found to be activated on membrane excision, in a manner sensitive to tyrosine phosphatase inhibitors. In MAFs isolated from animals deficient in receptor protein tyrosine phosphatase (RPTP)zeta, activation of the maxi-anion channel was significantly slower and less prominent compared with that observed in wild-type MAFs; however, channel activation was restored by transfection of the RPTP zeta gene. Thus it is concluded that activation of the maxi-anion channel involves protein dephosphorylation mediated by protein tyrosine phosphatases that include RPTP zeta in mouse fibroblasts, but not in C127 cells.

• 出版日期2009-10