科研之友
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摘要
In the present study, immunoproteornic analysis was utilized to systemically characterize global autoantibody profiles in autoimmune hepatitis (AIH). Sera from 21 patients with AN and 15 healthy controls were analyzed for the antibody reactivity against the protein antigens of HepG2, a human hepatoma cell line. The lysates of HepG2 cells were separated by two-dimensional electrophoresis and then immunoblotted with each serum sample. Matrix-assisted laser desorption/ionization mass spectrometry or/and nanoelectrospray ionization MS/MS were then used to identify antigens, among which a bifunctional enzyme in mitochondrial, fumarate hydratase (FH), was further analyzed by ELISA using recombinant FH as a coating antigen. A total of 18 immunoreactive spots were identified as 13 proteins, 8 of which have not been reported in AIH. Immune reactivity to FH was detected in 66.67% of patients with AN, 19.35% of patients with primary biliary cirrhosis (PBC), 12.31 % of patients with chronic hepatitis B (CHB), 6.35% of patients with chronic hepatitis C (CHC), 11.32% of patients with systemic lupus erythematosus (SLE), and 3.57% of normal individuals. The differences of prevalence between AN patients and healthy controls as well as other diseases were of statistical significance (P < 0,001). These data demonstrate the serological heterogeneity in AN and suggest the diversity of the mechanisms underlying AIH. FH, recognized mainly in AN rather than in viral hepatitis and other autoimmune diseases, may have utility in improved diagnosis of AIH.
- 出版日期2008-5
- 单位中国人民解放军第三０二医院; 河南大学; 四川大学; 首都医科大学
