Antitumor activity of cytokine-induced killer cells against human lung cancer

作者：Kim Hwan Mook; Lim Jaeseung; Park Song Kyu; Kang Jong Soon; Lee Kiho; Lee Chang Woo; Lee Ki Hoon; Yun Mi Jung; Yang Kyu Hwan; Han Gyoonhee; Kwon Soon Woo; Kim Youngsoo; Han Sang Bae^*

来源：International Immunopharmacology, 2007, 7(13): 1802-1807.

DOI：10.1016/j.intimp.2007.08.016

• cytokine-induced killer cells
• lung cancer
• adoptive immunotherapy

Lung cancer is the leading cause of cancer-related death among men and women in the world. Despite the aggressive treatment with surgery, radiation and chemotherapy, the long term survival for lung cancer patients remains low. In this study, the anti-tumor activity of cytokine-induced killer (CIK) cells against human lung cancer was evaluated in vitro and in vivo. Although CD3(+)CD56(+) CIK cells were rare in fresh human peripheral blood mononuclear cells, they could expand more than 1000-fold on day 14 in the presence of anti-CD3 antibody plus IL-2. At an effector-target cell ratio of 30:1, CIK cells destroyed 98% of NCI-H460 human lung cancer cells, which was determined by the Cr-51-release assay. In addition, CIK cells at doses of 3 and 30 million cells per mouse inhibited 57% and 77% of NCI-H460 tumor growth in nude mouse xenograft assay, respectively. This study suggests that CD3+CD56+ CIK cells may be used as an adoptive immunotherapy for patients with lung cancer.

• 出版日期2007-12-15