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摘要
A simple, highly sensitive, and label-free impedimetric immunosensor for detection of microcystin-LR (MC-LR) has been developed by immobilizing anti-microcystin-LR on gold nanoparticle/L-cysteine coated electrode. Under optimal conditions, MC-LR could be determined with a detection limit of 1.82 x 10(-2) ng mL(-1) and a linearity between 0.05 and 300.0 ng mL(-1). Moreover, the immunosensor exhibited long-term stability, and good reproducibility of the signal could be obtained up to 42 times with an R. S. D. of 3.58%. The average recovery rate was in the range of 96.8 similar to 112.5%. In addition, the whole detection time was only 15 min.
- 出版日期2010
- 单位江南大学
