A novel filamentous phage, fs-2, of Vibrio cholerae O139

作者：Ikema M^*; Honma Y

来源：Microbiology-SGM, 1998, 144: 1901-1906.

• Vibrio cholerae 0139
• filamentous phage

A novel filamentous bacteriophage, fs-2. was isolated from Vibrio cholerae 0139 strain MDO14. The fs-2 phage was a long filamentous particle 1200 nm long and 7 nm wide. The purified phage formed a turbid plaque when spotted on a lawn of the host organisms. The plaque-formation activity was stable following heating to 70 degrees C but was inhibited by treatment with chloroform. fs-2 had a single-stranded DNA genome and was converted to a double-stranded replicative form in the host cell. Almost all V. cholerae 0139 and 01 EI Tor biotype strains tested were sensitive to the phage, but most 01 classical strains and non-01 non-0139 strains were resistant. The fs-2 genome comprised 8651 nucleotides containing nine open reading frames, five of which had predicted protein products partially homologous to the reported protein products of other filamentous phages. Although the extent: of the homology was not particularly high, the genetic organization of other filamentous phages appears to be preserved in fs-2. The phage was not integrated into the chromosome of its host, but a 715 nucleotide fragment located in the large intergenic region of fs-2 was highly homologous to a part of region RS2 (repetitive sequence 2) of the V. cholerae CTX Phi sequence which is speculated to be required for integration of the phage into the V. cholerae chromosome at a specific site.

• 出版日期1998-7