A biorefinery from Nannochloropsis sp microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass

作者：Nobre B P; Villalobos F; Barragan B E; Oliveira A C; Batista A P; Marques P A S S; Mendes R L; Sovova H; Palavra A F; Gouveia L^*

来源：Bioresource Technology, 2013, 135: 128-136.

DOI：10.1016/j.biortech.2012.11.084

• Nannochloropsis
• Oils
• Carotenoids
• Biohydrogen
• Biorefinely

The microalga Nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. The microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical CO2 extraction. The temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. The best operational conditions to extract 33 glipids/100 g(dry) biomass were found to be at 40 degrees C, 300 bar and a CO2 flow-rate of 0.62 g/min. The effect of adding a co-solvent (ethanol) was also studied. When supercritical CO2 doped with 20% (w/w) ethanol was used, it was possible to extract 45 g(lipids)/100 g(dry) (biomass) of lipids and recover 70% of the pigments. Furthermore, the remaining biomass after extraction was effectively used as feedstock to produce biohydrogen through dark fermentation by Enterobacter aerogenes resulting in a hydrogen production yield of 60.6 mL/g(dry) (biomass).

• 出版日期2013-5