Absence of chlamydial deoxyribonucleic acid from testicular and epididymal samples from men with obstructive azoospermia

作者：Sripada Sreebala^*; Amezaga Maria Rosario; Hamilton Mark; McKenzie Hamish; Templeton Allan; Bhattacharya Siladitya

来源：Fertility and Sterility, 2010, 93(3): 833-836.

DOI：10.1016/j.fertnstert.2008.10.063

• Chlamydia
• male infertility
• azoospermia

Objective: To identify Chlamydia trachomatis DNA by polymerase chain reaction in the upper genital tract of men with obstructive azoospermia compared with men seeking vasectomy reversal.
Design: Case-control study.
Setting: Tertiary referral center. Aberdeen Royal Infirmary, Aberdeen, United Kingdom.
Patient(s): Cases were men with idiopathic obstructive azoospermia, and controls were men with azoospermia secondary to vasectomy.
Intervention(s): Chlamydia trachomatis-specific DNA test by polymerase chain reaction oil testicular and epididymal biopsy samples, as well as epididymal aspirate.
Main Outcome Measure(s): Presence of Chlamydia trachomatis DNA.
Result(s): We did not detect the presence of Chlamydia trachomatis-specific DNA by polymerase chain reaction in the epididymis or testis of 36 asymptomatic men with obstructive azoospermia ( 14 cases, 22 controls).
Conclusion(s): Our hypothesis that unrecognized, asymptomatic chlamydial infection will lead to complete bilateral obstruction of the male genital tract remains unproven. (Fertil Steril (R) 2010;93:833-6.

• 出版日期2010-2
• 单位河北医科大学