Assessment of bacterial resistance to organic solvents using a combined confocal laser scanning and atomic force microscopy (CLSM/AFM)

作者：Kuyukina Maria S^*; Ivshina Irena B; Korshunova Irina O; Rubtsova Ekaterina V

来源：Journal of Microbiological Methods, 2014, 107: 23-29.

DOI：10.1016/j.mimet.2014.08.020

• Organic solvent tolerant bacteria
• Rhodococcus
• Viability testing
• Confocal laser scanning microscopy
• Atomic force microscopy
• Profilometry

Using combined confocal laser scanning and atomic force microscopy (CLSM/AFM), bacterial viability under organic solvent stress was assessed at single cell level. Solvent-exposed bacteria stained with the LIVE/DEAD BacLight fluoresced green or red, allowing viable and dead cell discrimination. However, with toluene, butanol and acetonitrile, dually fluorescent cells appeared having compromised cell membranes. Changes in size, surface/volume ratio and roughness were revealed as possible resistance mechanisms.

• 出版日期2014-12