A Colorimetric CMOS-Based Platform for Rapid Total Serum Cholesterol Quantification

作者：Al Rawhani Mohammed A^*; Cheah Boon Chong; Macdonald Alasdair Iain; Martin Christopher; Hu Chunxiao; Beeley James; Gouveia Luiz Carlos; Grant James P; Campbell Gordon; Barrett Michael P; Cumming David R S

来源：IEEE Sensors Journal, 2017, 17(2): 240-247.

DOI：10.1109/JSEN.2016.2629018

• Biosensor
• CMOS
• colorimetric
• diagnostics
• enzyme
• photodiode

Elevated cholesterol levels are associated with a greater risk of developing cardiovascular disease and other illnesses, making it a prime candidate for detection on a disposable biosensor for rapid point of care diagnostics. One of the methods to quantify cholesterol levels in human blood serum uses an optically mediated enzyme assay and a bench top spectrophotometer. The bulkiness and power hungry nature of the equipment limits its usage to laboratories. Here, we present a new disposable sensing platform that is based on a complementary metal oxide semiconductor process for total cholesterol quantification in pure blood serum. The platform that we implemented comprises readily mass-manufacturable components that exploit the colorimetric changes of cholesterol oxidase and cholesterol esterase reactions. We have shown that our quantification results are comparable to that obtained by a bench top spectrophotometer. Using the implemented device, we have measured cholesterol concentration in human blood serum as low as 29 mu M with a limit of detection at 13 mu M, which is approximately 400 times lower than average physiological range, implying that our device also has the potential to be used for applications that require greater sensitivity.

• 出版日期2017-1-15