Anti-cancer activity of Angelica gigas by increasing immune response and stimulating natural killer and natural killer T cells

作者：Kim Seo Hyun; Lee Sung Won; Park Hyun Jung; Lee Sang Hee; Im Won Kyun; Kim Young Dong; Kim Kyung Hee; Park Sang Jae; Hong Seokmann^*; Jeon Sung Ho^*

来源：BMC Complementary and Alternative Medicine, 2018, 18(1): 218.

DOI：10.1186/s12906-018-2277-7

• Angelica gigas
• Immuno-stimulatory polysaccharide fraction
• Activation of innate immunity
• NK cells
• NKT cells
• Anti-cancer activity

Background: The polysaccharide component of Angelica gigas induces immuno-stimulatory effects on innate immune cells. However, it is unclear whether A. gigas' adjuvant activity on the immune system can elicit anticancer responses.
Methods: A water-soluble immuno-stimulatory component of A. gigas was prepared. How this ISAg modulated the activation of innate immune cells such as dendritic cells (DCs) was examined. ISAg-induced cytotoxic activity via natural killer (NK) and NKT cells was also tested using a tumor-bearing mouse model.
Results: ISAg treatment induced nitric oxide (NO) production and cytokine gene expression involved in innate immune responses. ISAg activated macrophages and DCs to secrete cytokine IL-12, through the TLR4 signaling pathway. IL-12 plays a crucial role in ISAg-mediated NK and NKT cell activation. Thus, the anti-cancer activity of NK and NKT cells induced ISAg-mediated cytotoxicity of B16 melanoma cells in mice.
Conclusions: These results indicated that the natural ingredient, ISAg, has adjuvant activity to induce strong anti-cancer activity of NK and NKT cells in vivo.

• 出版日期2018-7-18