A Method for Monitoring and Controlling Reproducibility of Intensity Data in Complex Electrospray Mass Spectra: A Thermometer Ion-based Strategy

作者：Lecchi Paolo; Zhao Jinghua; Wiggins Wesley S; Chen Tzong Hao; Yip Ping F; Mansfield Brian C; Peltier John M^*

来源：Journal of the American Society for Mass Spectrometry, 2009, 20(3): 398-410.

DOI：10.1016/j.jasms.2008.10.021

Reproducibility in mass spectral data is important in both biomarker discovery and spectral database searching. We report a strategy, employing a series of substituted benzylpyridinium thermometer ions that can be used to monitor changes in performance of multiple aspects of an electrospray ionization source that impact the intensity axis of a spectrum. Performance attributes, which could confound even isotope-based quantification strategies, are readily assessed using a mixture of thermometer ions. Based on the observed behavior of the ions, a procedure is proposed for monitoring instrument performance and compensating for factors that affect reproducibility across both time and instruments.

• 出版日期2009-3