Neutrophil CD64 expression as an important diagnostic marker of infection and sepsis in hospital patients

作者：Farias Mariela Granero^*; de Lucena Natalia Pieruccini; Dal Bo Suzane; de Castro Simone Martins

来源：Journal of Immunological Methods, 2014, 414: 65-68.

DOI：10.1016/j.jim.2014.07.011

• CD64
• Infection
• Flow cytometry
• Diagnosis

Introduction: Infection and sepsis are major health problems. Therefore, the need for improved diagnostic indicators, as well as for better therapeutic monitors in the treatment of infection, remains, since the current diagnostic tools have low specificity and passed through minimal changes in the last two decades.
Objective: The aim of this study was to establish the correlation of neutrophil CD64 with indicators of infection and sepsis.
Methods: We established the correlation of the neutrophil CD64 expression with the following variables: complete white blood count, band count, neutrophils, C-reactive protein (CRP), cultures, flags released by automated hematology analyzers and clinical groups. Accordingly clinical groups were divided into two: patients "without clinical or laboratory evidence of infection or inflammatory process" and "clinical or laboratory evidence of a systemic inflammatory response (SIRS) and systemic sepsis" based upon identification of organisms by culture. We analyzed 93 whole blood samples anticoagulated with K(3)EDTA of patients admitted in the Intensive Care Unit (ICU) of a community hospital.
Results: The expression CD64 was statistically significant with clinical groups, flags, and neutrophils and was not significantly correlated with total count of white blood cells and cultures.
Conclusion: Our results indicate that high expression of CD64 is an indicator important in the diagnosis of infection and sepsis.

• 出版日期2014-12-1