Application of silver nanoparticles in the detection of SYBR Green I by surface enhanced Raman and surface-enhanced fluorescence

作者：Guo, Wei; Wu, Jian^*; Wang, Chunyan; Zhang, Tian; Chen, Tao

来源：Journal of Nanoparticle Research, 2018, 20(5): 122.

DOI：10.1007/s11051-018-4228-0

• Surface-enhanced Raman scattering
• Surface-enhanced fluorescence
• SYBR green I
• PCR
• Fluorescent nanostructured dye
• Biomedical visualization

Silver nanomaterials have remarkable application in biomedical detection due to their unique surface plasmon resonance (SPR) characteristics. It can be used for surface-enhanced Raman scattering (SERS) and surface-enhanced fluorescence (SEF). Current research elaborates a technique for improvement of SYBR Green I detection obtained from surface-enhanced Raman scattering (SERS) and surface-enhanced fluorescence (SEF) by silver nanoparticles with the average size about 70 nm. Primarily, SYBR Green I is an important fluorescent dye used in polymerase chain reaction (PCR). It is found that both Raman and fluorescence can be used for detection of this dye. Furthermore, the enhanced efficiency of the Raman and fluorescence by SERS and SEF is observed in this study, the enhancement factor for Raman signals is 3.2 x 10(3), and the fluorescence intensity bincreased two times by SEF. The quantitative detection of SYBR Green I by SERS and SEF can be achieved. The present work can be used to improve the detection of SYBR Green I by SERS and SEF. It would also be employed for high-sensitive detection of other materials in the future.

• 出版日期2018-4-29
• 单位北京工业大学