Notoginsenoside R1 inhibits oxidized low-density lipoprotein induced inflammatory cytokines production in human endothelial EA.hy926 cells

作者：Su, Ping; Du, Shijing; Li, Hang; Li, Zhi; Xin, Wenfeng; Zhang, Wensheng^*

来源：European Journal of Pharmacology, 2016, 770: 9-15.

DOI：10.1016/j.ejphar.2015.11.040

• Notoginsenoside R1
• Inflammatory cytokines
• Oxidized low-density lipoprotein
• Peroxisome proliferator-activated receptor gamma
• Endothelial cell

Notoginsenoside R1 (NG-R1), a unique and main active ingredient of Panax notoginseng, has been described to exhibit anti-inflammatory activity. However, its protective effects against oxidized low-density lipoprotein (oxLDL)-induced inflammatory injury in vascular endothelial cells have not been clarified. In the present study, we have evaluated the anti-inflammatory effects of NG-R1 on oxLDL-induced endothelial cells and its possible molecular mechanism of action. Our results showed that NG-R1 treatment significantly attenuated oxLDL-induced expression of tumor necrosis factor (TNE)-alpha and interleukin (IL)-1 beta. These effects were accompanied with suppression of oxLDL-induced activation of NF-kappa B and Mitogen-activated protein kinases (MAPK). Moreover, NG-R1 also increased in Peroxisome proliferator-activated receptor gamma (PPAR gamma) protein expression and transcription levels, and attenuated oxLDL-induced suppression of PPAR gamma expression. The inhibition of NG-R1 on oxLDL-induced TNF-alpha and IL-1 beta productions can be reversed by PPAR gamma antagonist GW9662. In conclusion, these data suggested that NG-R1 could suppress oxLDL-induced inflammatory cytokines production via activating PPAR gamma, which subsequently inhibiting oxLDL-induced NF-kappa B and MAPK activation.

• 出版日期2016-1-5
• 单位北京师范大学