Discussion on Validity of Rana maoershanensis Based on Partial Sequence of 16S rRNA Gene

作者：Yang Baotian; Lu Yuyan; Li Pipeng^*

来源：Asian Herpetological Research, 2010, 1(2): 97-102.

DOI：10.3724/SP.J.1245.2010.00097

• Rana maoershanensis
• validity
• variation
• phylogeny
• 16S rDNA

Rana maoershanensis found in Mt. Maoershan in Guangxi, China was reported as a new species in 2007, but there was no molecular data for this frog. The partial sequences (543 bp) of 16S rRNA gene from 12 specimens of 3 brown frog species (Rana hanluica, R. maoershanensis and R. chensinensis) were analyzed with 17 specimens of 9 species from GenBank. The nucleotide sequence divergence between R. maoershanensis and the other brown frog species were 4.5%-6.5%, with 22-30 nucleotide substitutions at this locus. The phylogenetic relationships based on MP, ML, and Bayesian inference indicate that the brown frogs from Southern China were diverged into three groups (Clades A, B and C). R. maoershanensis was clustered together a well-supported subclade (B-1). It is suggested that R. maoershanensis is a valid species.

• 出版日期2010-12-25
• 单位沈阳师范大学