Decreased coenzyme A levels in ridA mutant strains of Salmonella enterica result from inactivated serine hydroxymethyltransferase

作者：Flynn Jeffrey M; Christopherson Melissa R; Downs Diana M^*

来源：Molecular Microbiology, 2013, 89(4): 751-759.

DOI：10.1111/mmi.12313

The RidA/Yer057/UK114 family of proteins is well represented across the domains of life and recent work has defined both an in vitro activity and an in vivo role for RidA. RidA proteins have enamine deaminase activity, and in their absence the reactive 2aminoacrylate (2-AA) accumulates and inactivates at least some pyridoxal 5%26apos;-phosphate (PLP)-containing enzymes in Salmonella enterica. The conservation of RidA suggested that 2-AA was a ubiquitous cellular stressor that was generated in central metabolism. Phenotypically, strains of S. enterica that lack RidA accumulated significantly more pyruvate in the growth medium than wild-type strains. Here we dissected this ridA mutant phenotype and showed it was an indirect consequence of damage to serine hydroxymethyltransferase (GlyA; E. C. 2.1.2.1). The results here identified a fourth PLP enzyme as a target of enamine stress in Salmonella.

• 出版日期2013-8