High yield bio-butanol production by solvent-producing bacterial microflora

作者：Cheng Chieh Lun; Che Pei Yi; Chen Bor Yann; Lee Wen Jhy; Chien Liang Jung; Chang Jo Shu^*

来源：Bioresource Technology, 2012, 113: 58-64.

DOI：10.1016/j.biortech.2011.12.133

• Bio-butanol
• Clostridium
• Response surface methodology
• Butyric acid
• Pressurized fermentation

Highly efficient butanol-producing bacterial microflora were isolated from hydrogen-producing sludge of a sewage treatment plant. Based on denaturing gradient gel electrophoresis (DGGE) analysis and 16s rDNA comparison, four strains from the butanol-producing microflora were identified as Clostridium saccharoperbutylacetonicum, Clostridium butylicum, Clostridium beijernckii, and Clostridium acetobutylicum. The effects of glucose. FeSO4 center dot 7H(2)O and yeast extract concentrations on the butanol production by the mixture culture were investigated on batch mode. The medium composition for bio-butanol production was optimized using the Box-Behnken design and response surface methodology (RSM). The maximum butanol production rate (0.25 +/- 0.02 g/L-h) and concentration (12.4 g/L) were obtained under the condition of glucose concentration, 60 g/L; FeSO4 center dot 7H(2)O, 0.516 g/L; yeast extract concentration, 5.13 g/L. Addition of 6.0 g/L butyric acid significantly increased the butanol titer to 17.51 +/- 0.49 g/L. Pressurized fermentation strategy (employed with a 5 L fermentor) further enhanced the butanol concentration to 21.35 g/L, along with a maximum butanol rate of 1.25 g/L-h.

• 出版日期2012-6