Acute toxicity of chlorobenzenes in Tetrahymena: Estimated by microcalorimetry and mechanism

作者：Zhang, Tian; Li, Xi^*; Min, Xinmin; Fang, Tingting; Zhang, Zhijun; Yang, Lu; Liu, Peng

来源：Environmental Toxicology and Pharmacology, 2012, 33(3): 377-385.

DOI：10.1016/j.etap.2012.01.009

• Chlorobenzene
• Tetrahymena
• Microcalorimetry
• Toxicity IC50
• Growth

The toxicity of chlorobenzenes to Tetrahymena growth metabolism was studied by microcalorimetty. The growth constant (k), peak time (T) and generation times (T-G) were calculated. IC50 of chlorobenzenes was obtained through the kinetic parameters. The results suggested that the order of toxicity was 1,2,4-trichlorobenzene > o-dichlorobenzene > p-dichlorobenzene > m-dichlorobenzene > chlorobenzene. ATR-FTIR spectra revealed that amide groups and PO2- of the phospholipid phospho-diester, both in the hydrophobic end exposed to the outer layer, were the easiest to be damaged. The relationship between IC50 and chemicals structure parameters (E-LUMO, E-HOMO, logK(OW), Sigma Q(R), Delta Q pi(R) and Delta E), indicated that the more chlorine atoms were substituted, the greater the toxicity was. Chlorobenzenes have toxicity of non-polar narcosis. Their toxicity is proportional to their concentrations at the site of action, and caused by membrane perturbation.

• 出版日期2012-5
• 单位武汉理工大学