Determination of Morphine in Pharmaceutical Products by On-Line Solid-Phase Extraction and High-Performance Liquid Chromatography

作者：Yang, Yan-Ling; Ye, Xiao-Xue; Li, Yu-Sang^*; Tan, Zhi-Fang; Jiang, Ji-Zhou^*

来源：Analytical Letters, 2016, 49(9): 1303-1309.

DOI：10.1080/00032719.2015.1101603

• Double-ternary gradient high-performance liquid chromatography
• morphine
• traditional Chinese medicine
• solid-phase extraction

A method for the rapid determination of morphine in Chinese medicines was developed using on-line solid-phase extraction combined with double-ternary gradient liquid chromatography. Licorice tablets and camphor tincture were used as representative examples of traditional Chinese medicine. The results indicated that morphine provided a linear response from 0.0239 to 0.239m g mL(-1). The average recovery was as high as 99% with a relative standard deviation of 0.9%. Moreover, compared with conventional off-line solid-phase extraction and liquid chromatography, this approach offered a significant reduction in the analysis time. This method may provide for more efficient determination of morphine in traditional Chinese medicine.

• 出版日期2016
• 单位中南民族大学; 华中科技大学; 中国科学院武汉物理与数学研究所