Does RecA have a role in Borrelia recurrentis?

作者：Cutler S J^*; Rinky I J; Bonilla E M

来源：Clinical Microbiology and Infection, 2011, 17(2): 195-197.

DOI：10.1111/j.1469-0691.2010.03249.x

• Borrelia duttonii
• Borrelia recurrentis
• louse-borne relapsing fever
• RecA
• relapsing fever
• tick-borne relapsing fever

P>Genomic sequencing of two relapsing fever spirochaetes showed truncation of recA in Borrelia recurrentis, but not in Borrelia duttonii. RecA has an important role among bacteria; we investigated whether this characteristic was representative of B. recurrentis, or an artefact following in vitro cultivation. We sequenced recA directly from samples of patient with louse-borne relapsing fever (B. recurrentis) or tick-borne relapsing fever (B. duttonii). We confirmed the premature stop codon in seven louse-borne relapsing fever samples, and its absence from three tick-borne relapsing fever samples. Furthermore, specific signature polymorphisms were found that could differentiate between these highly similar spirochaetes.

• 出版日期2011-2