Aflatoxin exposure in Nigerian children with severe acute malnutrition

作者：McMillan Amy; Renaud Justin B; Burgess Kevin M N; Orimadegun Adebola E; Akinyinka Olusegun O; Allen Stephen J; Miller J David; Reid Gregor; Sumarah Mark W^*

来源：Food and Chemical Toxicology, 2018, 111: 356-362.

DOI：10.1016/j.fct.2017.11.030

• Aflatoxin
• Severe acute malnutrition
• Aflatoxin-lysine adduct
• High resolution LC-MS/MS
• Isotope dilution mass spectrometry
• Kwashiorkor

Aflatoxin exposure is an important public health concern in sub-Saharan Africa as well as parts of Latin America and Asia. In addition to hepatocellular carcinoma, chronic aflatoxin exposure is believed to play a role in childhood growth impairment. The most reliable biomarker of chronic aflatoxin exposure is the aflatoxin-albumin adduct, as measured by ELISA or isotope dilution mass spectrometry (IDMS). In this report, we have used high resolution LC-MS/MS with IDMS to quantitate AFB(1)-lysine in an extremely vulnerable population of Nigerian children suffering from severe acute malnutrition. To increase the sensitivity and reliability of the analyses, a labelled AFB(1)-(C6N2)-C-13-N-15-lysine internal standard was synthesized. AFB(1)-lysine concentrations in this population ranged between 0.2 and 59.2 pg/mg albumin, with a median value of 2.6 pg/mg albumin. AFB(1)-lysine concentrations were significantly higher in stunted children (median = 4.6 pg/mg) compared to non stunted (1.2 pg/mg), as well as in children with severe acute malnutrition (4.3 pg/mg) compared to controls (0.8 pg/mg). The median concentrations were also higher in children with kwashiorkor (6.3 pg/mg) compared to those suffering from marasmus (0.9 pg/mg). This is the first report of the use of high-resolution mass spectrometry to quantitate AFB(1)-lysine in humans.

• 出版日期2018-1