Diverse human extracellular RNAs are widely detected in human plasma

作者：Freedman Jane E^*; Gerstein Mark; Mick Eric; Rozowsky Joel; Levy Daniel; Kitchen Robert; Das Saumya; Shah Ravi; Danielson Kirsty; Beaulieu Lea; Navarro Fabio C P; Wang Yaoyu; Galeev Timur R; Holman Alex; Kwong Raymond Y; Murthy Venkatesh; Tanriverdi Selim E; Koupenova Zamor Milka; Mikhalev Ekaterina; Tanriverdi Kahraman

来源：Nature Communications, 2016, 7(1): 11106.

DOI：10.1038/ncomms11106

There is growing appreciation for the importance of non-protein-coding genes in development and disease. Although much is known about microRNAs, limitations in bioinformatic analyses of RNA sequencing have precluded broad assessment of other forms of small-RNAs in humans. By analysing sequencing data from plasma-derived RNA from 40 individuals, here we identified over a thousand human extracellular RNAs including microRNAs, piwi-interacting RNA (piRNA), and small nucleolar RNAs. Using a targeted quantitative PCR with reverse transcription approach in an additional 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence in plasma of many non-microRNA small-RNAs was confirmed in an independent cohort. We present comprehensive data to demonstrate the broad and consistent detection of diverse classes of circulating non-cellular small-RNAs from a large population.

• 出版日期2016-4
• 单位NIH