Perturbations in intracellular Ca2+ handling in skeletal muscle in the G93A*SOD1 mouse model of amyotrophic lateral sclerosis

作者：Chin Eva R^*; Chen Dapeng; Bobyk Kostyantyn D; Mazala Davi A G

来源：American Journal of Physiology - Cell Physiology, 2014, 307(11): C1031-C1038.

DOI：10.1152/ajpcell.00237.2013

• skeletal muscle
• intracellular Ca2+
• excitation-contraction coupling
• amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by skeletal muscle atrophy and weakness, ultimately leading to respiratory failure. The purpose of this study was to assess changes in skeletal muscle excitation-contraction (E-C) coupling and intracellular Ca2+ handling during disease progression in the G93A*SOD1 ALS transgenic (ALS Tg) mouse model. To assess E-C coupling, single muscle fibers were electrically stimulated (10-150 Hz), and intracellular free Ca2+ concentration was assessed using fura-2. There were no differences in peak fura-2 ratio at any stimulation frequency at 70 days (early presymptomatic). However, at 90 days (late presymptomatic) and 120-140 days (symptomatic), fura-2 ratio was increased at 10 Hz in ALS Tg compared with wild-type (WT) fibers (0.670 +/- 0.02 vs. 0.585 +/- 0.02 for 120-140 days; P < 0.05). There was also a significant increase in resting fura-2 ratio at 90 days (0.351 +/- 0.008 vs. 0.390 +/- 0.009 in WT vs. ALS Tg; P < 0.05) and 120-140 days (0.374 +/- 0.001 vs. 0.415 +/- 0.003 in WT vs. ALS Tg; P < 0.05). These increases in intracellular Ca2+ in ALS Tg muscle were associated with reductions in the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase proteins SERCA1 (to 54% and 19% of WT) and SERCA2 (to 56% and 11% of WT) and parvalbumin (to 80 and 62% of WT) in gastrocnemius muscle at 90 and 120-140 days, respectively. There was no change in dihydropyridine receptor/L-type Ca2+ channel at any age. Overall, these data demonstrate minimal changes in electrically evoked Ca2+ transients but elevations in intracellular Ca2+ attributable to decreased Ca2+-clearance proteins. These data suggest that elevations in cellular Ca2+ could contribute to muscle weakness during disease progression in ALS mice.

• 出版日期2014-12-1