Multifunctional Biocompatible Graphene Oxide Quantum Dots Decorated Magnetic Nanoplatform for Efficient Capture and Two-Photon Imaging of Rare Tumor Cells

作者：Shi Yongliang; Pramanik Avijit; Tchounwou Christine; Pedraza Francisco; Crouch Rebecca A; Chavva Suhash Reddy; Vangara Aruna; Sinha Sudarson Sekhar; Jones Stacy; Sardar Dhiraj; Hawker Craig; Ray Paresh Chandra^*

来源：ACS Applied Materials & Interfaces, 2015, 7(20): 10935-10943.

DOI：10.1021/acsami.5b02199

• luminescent magnetic nanoplatform
• graphene oxide quantum dots
• highly efficient two-photon-absorbing material
• rare liver cancer cell separation from blood
• selective two-photon imaging

Circulating tumor cells (CTCs) are extremely rare cells in blood containing billions of other cells. The selective capture and identification of rare cells with sufficient sensitivity is a real challenge. Driven by this need, this manuscript reports the development of a multifunctional biocompatible graphene oxide quantum dots (GOQDs) coated, high-luminescence magnetic nanoplatform for the selective separation and diagnosis of Glypican-3 (GPC3)-expressed Hep G2 liver cancer tumor CTCs from infected blood. Experimental data show that an anti-GPC3-antibody-attached multifunctional nanoplatform can be used for selective Hep G2 hepatocellular carcinoma tumor cell separation from infected blood containing 10 tumor cells/mL of blood in a 15 mL sample. Reported data indicate that, because of an extremely high two-photon absorption cross section (40530 GM), an anti-GPC3-antibody-attached GOQDs-coated magnetic nanoplatform can be used as a two-photon luminescence platform for selective and very bright imaging of a Hep G2 tumor cell in a biological transparency window using 960 nm light. Experimental results with nontargeted GPC3(-) and SK-BR-3 breast cancer cells show that multifunctional-nanoplatform-based cell separation, followed by two-photon imaging, is highly selective for Hep G2 hepatocellular carcinoma tumor cells.

• 出版日期2015-5-27