Antigenotoxic Effects of the Disulfide Compound Persicasulfide A (PSA) on Rat Lymphocytes Exposed to Oxidative Stress

作者：Noroozi Saeid; Mosaffa Fatemeh; Soltani Fatemeh; Iranshahi Mehrdad; Karimi Gholamreza; Malekaneh Mohammad; Haghighi Fatemeh; Behravan Javad^*

来源：Planta Medica, 2009, 75(1): 32-36.

DOI：10.1055/s-0028-1088360

• Ferula persica
• Apiaceae
• antigenotoxicity
• comet assay
• persicasulfide A

The antigenotoxic effect of persicasulfide A (PSA) from Ferula persica on DNA damage induced by hydrogen peroxide (H(2)O(2)) was evaluated using single cell gel electrophoresis (SCGE). PSA was extracted from F. persica, characterized by NMR and its antioxidant/antigenotoxic effects were investigated. The antigenotoxic effect of solutions containing either PSA (1, 10, 50, 100, 200, 300, 400 and 500 mu M) or ascorbic acid (250, 500, 750 and 1000 mu M) alone, or in the presence of H(2)O(2) (25, 50, 100 and 200 mu M) were tested on lymphocytes derived from the blood of healthy male Wistar rats (250-300 g) by using the comet assay. The degree of damage to DNA after exposure to different solutions was calculated based on the amount of DNA present in the tail compared to the total amounts of lymphocyte DNA. PSA did not show genotoxicity and caused a 50% reduction in DNA damage induced by H(2)O (EC(50): 476.47 +/- 67.46 mu M). Compared to the EC(50) for ascorbic acid (1399.23 +/- 205.21 mu M), it was deduced that PSA was more effective than ascorbic acid in the prevention of oxidative damage to DNA.

• 出版日期2009-1