Dynamic phosphorylation of Histone Deacetylase 1 by Aurora kinases during mitosis regulates zebrafish embryos development

作者：Loponte Sara; Segre Chiara V; Senese Silvia; Miccolo Claudia; Santaguida Stefano; Deflorian Gianluca; Citro Simona; Mattoscio Domenico; Pisati Federica; Moser Mirjam A; Visintin Rosella; Seiser Christian; Chiocca Susanna^*

来源：Scientific Reports, 2016, 6(1): 30213.

DOI：10.1038/srep30213

Histone deacetylases (HDACs) catalyze the removal of acetyl molecules from histone and nonhistone substrates playing important roles in chromatin remodeling and control of gene expression. Class I HDAC1 is a critical regulator of cell cycle progression, cellular proliferation and differentiation during development; it is also regulated by many post-translational modifications (PTMs). Herein we characterize a new mitosis-specific phosphorylation of HDAC1 driven by Aurora kinases A and B. We show that this phosphorylation affects HDAC1 enzymatic activity and it is critical for the maintenance of a proper proliferative and developmental plan in a complex organism. Notably, we find that Aurora-dependent phosphorylation of HDAC1 regulates histone acetylation by modulating the expression of genes directly involved in the developing zebrafish central nervous system. Our data represent a step towards the comprehension of HDAC1 regulation by its PTM code, with important implications in unravelling its roles both in physiology and pathology.

• 出版日期2016-7-26