Derivation of a valid momentary first-order rate constant for kinetic and energetic analyses of enzymatic reactions

作者：Imoto Taiji^*

来源：Journal of Biochemistry, 2016, 160(6): 381-389.

DOI：10.1093/jb/mvw049

• energetics/energy
• barrier/enzyme
• kinetics/first order/transition state

To analyze enzymatic reactions energetically for comparison with non-enzymatic reactions (first order) under the same dimension, a method to derive valid momentary first-order rate constants for enzymatic reactions was developed. The momentary first-order rate constant, k(enz0) = k(cat[)ESl]e(,0)/[S](0), was derived for an enzymatic reaction under a certain condition. It was shown that this rate constant is applicable for a wide range of enzymatic reactions. Utilizing this constant, one can conduct reliable kinetic and energetic analyses of enzymatic reactions.

• 出版日期2016-12