A mechanism underlying AMPA receptor trafficking during cerebellar long-term potentiation

作者：Kakegawa W; Yuzaki M^*

来源：Proceedings of the National Academy of Sciences, 2005, 102(49): 17846-17851.

DOI：10.1073/pnas.0508910102

• cerebellum
• glutamate receptor
• long-term depression
• nitric oxide

Long-term potentiation (LTP) is mediated by the activity-driven delivery of GluR1 glutamate receptors via Ca2+/calmodulin-dependent protein kinase 11 activity in various brain regions. Recently, postsynaptic LTIP was shown to be induced at parallel fiber-Purkinje cell synapses by stimulating the parallel fibers at 1 Hz or applying a NO donor. Here, we demonstrate that NO-evoked postsynaptic LTP in mice cerebellum was blocked by botulinum toxin and enhanced by prior treatment with phorbol ester, which is known to induce GluR2 endocytosis. Interestingly, such LTP was not affected by a Ca2+/calmodulin-dependent protein kinase 11 inhibitor or a peptide binding to a protein interacting with C kinase 1, but was blocked by a peptide binding to N-ethylmaleimide-sensitive factor, which specifically binds to GluR2. Therefore, although the synaptic incorporation of GluR2 has been reported to be a constitutive pathway, NO-induced postsynaptic LTP in Purkinje cells is likely mediated by a pathway involving N-ethylmaleimide-sensitive factor-dependent GluR2 trafficking.

• 出版日期2005-12-6