科研之友
微信
新浪微博
Facebook
分享链接
摘要
The aim of the present study was to examine the expression and significance of CCAAT/enhancer binding protein a (C/EBPa) and SRY-related high mobility group box containing transcription factor 4 (SOX4) in chronic myeloid leukemia (CML). Bone marrow samples were collected from patients with CML, and peripheral blood mononuclear cells were collected from healthy controls. Protein and mRNA were extracted from the collected samples, and analyzed using western blotting and reverse transcription-quantitative polymerase chain reaction analyses, respectively. Spearman's method was used to evaluate the correlation between the expression levels of these two genes, with P<0.05 considered to indicate a statistically significant difference. A total of 79 patients, including 57 patients with newly diagnosed CML and 22 patients treated with imatinib therapy, and 30 controls were enrolled. The expression of SOX4 was upregulated in the patients with CML, whereas the expression of C/EBPa was downregulated (P<0.05). However, no differences were observed among the chronic, accelerated and blastic CML phases, respectively (P>0.05). In addition, no associations were found between the changes in expression and age, gender, white blood cells or the expression of breakpoint cluster region/abelson in patients (P>0.05). However, the expression of SOX4 was negatively correlated with the expression of C/EBPa (P<0.01). Following imatinib treatment, the expression of SOX4 was downregulated in the progression-free patients, but upregulated in the blastic phase patients, whereas the expression of C/EBPa showed the opposite trend. Therefore, C/EBPa and SOX4 were important and negatively associated with the process of CML, and the C/EBPa-SOX4 axis may be a novel potential therapeutic target for the treatment of CML.
- 出版日期2017-11
- 单位青岛大学; 烟台毓璜顶医院
