科研之友
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摘要
Mechanically scratching cell monolayers relieves contact inhibition and induces surviving cells near the wound edge to move and proliferate, The present work was designed to test whether surviving cells passively respond to newly available space, or whether cells are actively stimulated by signals from injured cells nearby, We monitored intracellular free Ca2+ ([Ca2+](i)) while scratching confluent monolayers of bovine pulmonary endothelial cells and mouse mammary epithelial cells. Within seconds after wounding, a transient elevation of [Ca2+](i) was observed in surviving cells, In endothelial cells, the [Ca2+](i) elevation propagated into the monolayer for a distance of 10 to 12 cell rows at a speed of 20 to 28 mu m/second, The amplitude of the wave of [Ca2+](i) was reduced as it propagated into the monolayer, but the velocity of the wave was nearly constant, Cells that experienced the [Ca2+](i) elevation had intact plasma membranes, and survived for over 24 hours post wounding, Removing extracellular Ca2+ decreased the amplitude by two-thirds and reduced the propagation rate by half, suggesting that Ca2+ influx contributed to the increased [Ca2+](i). To determine how [Ca2+](i) waves were stimulated, we blocked extracellular communication by fluid perfusion or intercellular communication by breaks in the monolayer.
- 出版日期1997-2
